Nε-(Carboxymethyl)lysine and Coronary Atherosclerosis-Associated Low Density Lipoprotein Abnormalities in Type 2 Diabetes: Current Status

In comparison to the general population, individuals with diabetes suffer a 3- to 4-fold increased risk for developing complications of atherosclerosis and vascular insufficiency. This fact should be taken into account to develop a suitable determinant for the early detection of these complications and subsequently reduce the adverse effect of type 2 diabetes. In vitro experiments have shown that the products of glucose auto-oxidation and Amadori adducts are both potential sources of Nε-(carboxymethyl)lysine (CML). Excessive formation of CML on low density lipoprotein (LDL) has been proposed to be an important mechanism for the dyslipidemia and accelerated atherogenesis observed in patients with type 2 diabetes. It has been postulated that the uptake of CML-LDL by LDL receptors is impaired, thereby decreasing its clearance from the blood circulation. Alternatively, the uptake of these modified LDL particles by scavenger receptors on macrophages and vascular smooth muscle cells (SMCs) and by AGE receptors on endothelial cells, SMCs, and monocytes is highly enhanced and this, in turn, is centrally positioned to contribute to the pathogenesis of diabetic vascular complications especially coronary artery disease. The present review summarizes the up-to-date information on effects and mechanism of type 2 diabetes-associated coronary atherosclerosis induced by CML-LDL modification

Role of Nε-(Carboxymethyl)Lysine in the Development of Ischemic Heart Disease in Type 2 Diabetes Mellitus

This study aims to determine the levels of Nε-(carboxymethyl)lysine (CML) in patients with Type 2 diabetic patients with and without ischemic heart disease (IHD) and to find for a possible association between circulating CML and a number of clinical parameters including lipids, hemoglobin A1c (HbA1c) and malondialdehyde (MDA) in Type 2 diabetic IHD patients. Serum CML levels were measured by enzyme-linked immunosorbent assay using polyclonal anti-CML antibodies. Serum levels of CML and MDA were assessed in 60 IHD patients with Type 2 diabetes, 43 IHD patients without Type 2 diabetes, 64 Type 2 diabetics without IHD, and 80 sex- and age-matched healthy subjects. Correlations studies between CML levels and lipids, HbA1c, and lipid peroxidation were performed in Type 2 diabetes patients with and without IHD. A statistical significance was observed in the levels of serum glucose, lipids (triglyceride, total cholesterol, HDL-cholesterol), MDA, HbA1c, CML and LDL-cholesterol (p<0.05) between the groups of the study. CML levels were significantly increased in diabetic IHD patients compared with Type 2 diabetes patients but without IHD (537.1 ± 86.1 vs 449.7 ± 54.9, p<0.001). A positive correlation was observed between serum levels of CML and MDA, r = 0.338 (p = 0.008) in Type 2 diabetes patients with IHD. However, age, HbA1c and lipids had no significant influence on CML levels among diabetics (p>0.05). In conclusion, this study demonstrates the effect of both diabetes and oxidative stress on the higher levels of circulating CML. These results showed that increased serum levels of CML are associated with the development of IHD in Type 2 diabetes mellitus

Prevalence and risk factors for child labour and violence against children in Egypt using Bayesian geospatial modelling with multiple imputation

Background The incidence of child labour, especially across developing nations, is of global concern. The use of children in employment in developing economies constitutes a major threat to the societies, and concerted efforts are made by the relevant stakeholders towards addressing some of the factors and issues responsible. Significant risk factors include socio-demographic and economic factors such as poverty, neglect, lack of adequate care, exposure of children to various grades of violence, parental education status, gender, place of residence, household size, residence type or size, wealth index, parental survivorship and household size. Egypt is the largest country in Africa by population. Although UNCIF 2017 reported that the worst forms of child labour in Egypt are concentrated in domestic work, forced begging and commercial sexual exploitation, the situation has received little attention. There are still very few studies initiated specifically to look at child labour in domestic service in Egypt and those that exist have been limited in the scope of their methodology. Geographical coverage and research for child labour in Egypt is also limited, as are accurate statistics and data. There was, therefore, a strong case for looking again at the domestic child labour phenomenon in Egypt, especially after the Demographic Health Survey (DHS) released the first data about child labour in Egypt in 2014. This study builds on the few findings of earlier work, and broadens coverage by including advanced methods and geographical effects of this problem. Objectives This study focuses on identifying socio-demographic, economic and geospatial factors associated with child labour participation. Methods We used the 2014 Egypt Demographic and Health Survey (EDHS) from the Ministry of Health and Population in Egypt, with the record of 20,560 never-married children aged 5–17 years engaging in economic activities, in and out of their home. The data focused on demographic and socio-economic characteristics of household members. Multivariate Bayesian geo-additive models were employed to examine the demographical and socio-economic factors for children working less than 16 hrs; between 16 and less 45 hrs; and over 45 hrs weekly. Results The results showed that at least 31.6% of the children in the age group from 5–10 were working, 68.5% of children aged 11–17 years were engaged in child labour for a wage, and 44.7% of the children in the age group from 5–10 were engaged in hazardous work. From the multivariate Bayesian geo-additive models, female children (with male children as reference category) working at least 16 hrs (OR: 1.3; with 95% CI: 1.2–1.5) were more likely to be engaged in child labour than girls working 16 to 45 hrs (OR: 1; 95% CI: 0.3–1.5). Children born to women without formal education, in non-hazardous jobs, irrespective of the hours spent at work, were more likely to be involved in child labour (52.9%, 56.8%, 62.4%) compared to children of mothers with some level of education. Finally, children who have experienced psychological aggression and physical punishment are more likely to be used as child labour than those without such experience across the job types and hours spent. North-eastern Egypt has a higher likelihood of child labour than most other regions, while children who live in the Delta are more engaged in hazardous work. Conclusion This study revealed a significant influence of socio-demographic and economic factors on child labour and violence against children in Egypt. Poverty, neglect, lack of adequate care and exposure of children to various grades of violence are major drivers of child labour across the country. The spatial effect suggests the need to give more attention to some areas that have high rates of child labour, such as the Nile Delta, Upper Egypt, and North-eastern Egypt

Kinetics of the electropolymerization of aminoanthraquinone from aqueous solutions and analytical applications of the polymer film

AbstractPoly 1-amino-9, 10-anthraquinone (PAAQ) films were prepared by the electropolymerization of 1-amino-9,10-anthraquinone (AAQ) on platinum substrate from aqueous media, where 5.0×10−3molL−1 AAQ and 6.0molL−1 H2SO4 were used. The kinetics of the electropolymerization process was investigated by determining the change of the charge consumed during the polymerization process with time at different concentrations of both monomer and electrolyte. The results have shown that the process follows first order kinetics with respect to the monomer concentration. The order of the reaction with respect to the aqueous solvent i.e. H2SO4 was found to be negative. The polymer films were successfully used as sensors for the electroanalytical determination of many hazardous compounds, e.g. phenols, and biologically important materials like dopamine. The electroanalytical determination was based on the measurements of the oxidation current peak of the material in the cyclic voltammetric measurements. The cyclic voltammograms were recorded at a scan rate of 100mVs−1 and different analyte concentrations. A calibration curve was constructed for each analyte, from which the determination of low concentrations of catechol and hydroquinone (HQ) as examples of hazardous compounds present in waste water and also for ascorbic acid and dopamine as examples of valuable biological materials can be achieved

A Case report: Rare Complication of a common procedure- Pneumocephalus after Epidural Injection

Our patient 26 yrs. an old female patient 47 kg, ASA II , GDM on insulin was given lumbar epidural for labor pain relief using loss of resistance to air technique with 3 ml of air. After 10 h, she developed sudden loss of consciousness. An urgent CT scan was done which revealed pneumocephalus. Our goal is to report the case of a patient who presented Pneumocephalus after Epidural Injection

Application of Cardiovascular disease (CVD) risk assessment equations to the Thai population

Objective: The objectives of this study are: 1.) To calculate the probability of Cardiovascular Disease (CVD) events by applying three different equations, which are: the Asia-Pacific Cohort Study (APCS) equation, the Framingham-Asia equation and the original Framingham equation, to the individual risk factors data from the NHESIV, Thailand. 2) To estimate the number of 8-10 years CVD events. 3) To validate and identify the most suitable CVD risk equations for the Thai population. The individual risk factors from the NHESIV dataset was entered into a Microsoft Excel spreadsheet as the baseline population. Methods: Asia-Pacific Collaborative Cohort Study (APCCS) equations, the Framingham-Asia equation and the original Framingham equation, are applied to calculate the probability of 8 to 10 years CVD events by age groups and gender. The CVD events in this analysis refer to all fatal and non-fatal CVD events (ICD10, I00-I99), which include Ischemic heart disease (IHD) (ICD10, I20-I25) and stroke (ICD10, I60-I69). Results: The 4th National Health Examination Survey IV 2009 (NHESIV) dataset has been entered into a Microsoft Excel spreadsheet as the baseline population. APCCS, the Framingham-Asia and the original Framingham equations, were applied to the NHESIV dataset. The APCCS equation calculated the average 8-years probability of getting CVD as 8.3% in men and 7.8% in women. The 8-year likelihood of CVD in the Framingham-Asia equation was 7.2% in men and 8.1% in women. The original Framingham equation showed the highest probability of 10-years CVD which were 18.8% in men and 11.1% in women. Conclusions: The original Framingham equation overestimated the risk of CVD in the Thai population in all age groups. The Asia-Pacific Cohort Study (APCCS) and the Framingham-Asia equations, both performed better estimation than the original Framingham equation in both men and women

Wound Healing Activities of Rafflesia Hasseltii Extract in Rats

The effects of topical application of Rafflesia hasseltii buds and flowers extract on the rate of wound healing and histology of healed wound were assessed. Four groups of adult male Sprague Dawley rats were experimentally wounded in the posterior neck area. A thin layer of blank placebo was applied topically to wounds of Group 1 rats. Wounds of experimental animals (Group 2 and 3) were treated with placebo containing 5% and 10% R. hasseltii buds extract, respectively. A thin layer of Intrasite gel was applied topically to wounds of Group 4 animals as reference. Macroscopically, wounds treated with placebo containing 5% and 10% R. hasseltii buds extract or Intrasite gel have been significantly accelerated the rate of wound healing compared to placebo-treated wounds. Histological analysis of healed wounds has confirmed this effect. Wounds treated with placebo containing 5%, 10% R. hasseltii buds extract or Intrasite gel showed markedly less scar width at wound enclosure and granulating tissue contained markedly more collagen and proliferating fibroblasts, but with the absence of inflammatory cells compared to wounds treated with blank placebo. In conclusion, the findings of increased rate of wound closure and contraction together with the histological findingssuggest that Rafflesia hasseltii buds extract is very effective in accelerating the wound healing process

Survey on Machine Learning Algorithms Enhancing the Functional Verification Process

The continuing increase in functional requirements of modern hardware designs means the traditional functional verification process becomes inefficient in meeting the time-to-market goal with sufficient level of confidence in the design. Therefore, the need for enhancing the process is evident. Machine learning (ML) models proved to be valuable for automating major parts of the process, which have typically occupied the bandwidth of engineers; diverting them from adding new coverage metrics to make the designs more robust. Current research of deploying different (ML) models prove to be promising in areas such as stimulus constraining, test generation, coverage collection and bug detection and localization. An example of deploying artificial neural network (ANN) in test generation shows 24.5× speed up in functionally verifying a dual-core RISC processor specification. Another study demonstrates how k-means clustering can reduce redundancy of simulation trace dump of an AHB-to-WHISHBONE bridge by 21%, thus reducing the debugging effort by not having to inspect unnecessary waveforms. The surveyed work demonstrates a comprehensive overview of current (ML) models enhancing the functional verification process from which an insight of promising future research areas is inferred

Association of plasminogen activator inhibitor-1 and tissue plasminogen activator with type 2 diabetes and metabolic syndrome in Malaysian subjects

<p>Abstract</p> <p>Background</p> <p>Increased plasma plasminogen activator inhibitor-1 (PAI-1) activity and decreased tissue plasminogen activator (tPA) activity could be considered a true component of the metabolic syndrome (MetS) associated with an increased risk of developing cardiovascular diseases (CVD) and fibrinolytic abnormalities. The aim of this study was to investigate the association of tPA and its inhibitor PAI-1 with type 2 diabetes (T2D) and MetS and interrelationship between PAI-1and tPA activities and antigens in Malaysian T2D and normal subjects.</p> <p>Methods</p> <p>The plasma activities and antigens of PAI-1 and tPA and the levels of the tPA/PAI-1 complex as well as serum insulin, parameter of the coronary risk panel and plasma glucose at fasting state were studied in 303 T2D subjects (227 with MetS and 76 without MetS), 131 normal non-diabetic non-metabolic subjects and 101 non-diabetic MetS subjects.</p> <p>Results</p> <p>The PAI-1 activity was higher in subjects with T2D with MetS (P = 9.8 × 10^-19) and non-diabetic subjects with MetS (P = 3.0 × 10^-15), whereas the tPA activity was lower in T2D with MetS (P = 0.003) as compare to normal subjects. Plasma tPA antigen levels were higher in subjects with T2D with MetS (P = 8.9 × 10^-24), T2D without MetS (P = 1.3 × 10^-13) and non-diabetic MetS subjects (P = 0.002). The activity and antigen of PAI-1 in normal subjects were related to insulin resistance (P = 2.2 × 10^-4; 0.007). Additionally, the PAI-1 activity was associated with an increased waist circumference (P = 2.2 × 10^-4) and decreased HDL-c (P = 0.005), whereas the tPA activity was associated with decreased FBG (P = 0.028). The highest correlation was between PAI-1 activity and its antigen (R²= 0.695, P = 1.1 × 10^-36) in diabetic subjects. The tPA activity negatively correlated with its antigen (R²= -0.444, P = 7.7 × 10^-13) in normal subjects and with the PAI-1 activity and antigen (R²= -0.319, P = 9.9 × 10^-12; R2 = -0.228, P = 3.4 × 10^-6) in diabetic subjects.</p> <p>Conclusions</p> <p>PAI-1 and tPA activities and antigens were associated with diabetes and MetS parameters in Malaysian subjects.</p

Effect of Plasminogen Activator Inhibitor-1 and Tissue Plasminogen Activator Polymorphisms on Susceptibility to Type 2 Diabetes in Malaysian Subjects

Elevated activity of plasminogen activator inhibitor-1 (PAI-1) and decreased tissue plasminogen activator (tPA) activity are considered to be important risk factors for type 2 diabetes mellitus (T2DM) and metabolic syndrome (MetS). The aim of this study was to investigate the association of the PAI-1 4G/5G and tPA Alu-repeat I/D polymorphisms with T2DM in Malaysian subjects. Serum insulin, coronary risk panel, plasma glucose, and PAI-1 4G/5G and tPA Alu-repeat I/D polymorphisms were studied in 303 T2DM subjects (227 with MetS and 76 without MetS) and 131 normal subjects without diabetes and MetS. Statistical analysis showed that the dominant and additive models of PAI-1 4G/5G polymorphism showed a weak association with T2DM without MetS (OR = 2.35, P = 0.045; OR = 1.67, P = 0.058). On the other hand, the recessive model of the tPA Alu-repeat I/D polymorphism showed an association with T2DM with MetS (OR = 3.32, P = 0.013) whereas the dominant and additive models of the tPA Alu-repeat I/D polymorphism were not associated with T2DM either with or without MetS